| 质粒名称: | pGWB455 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 74837 |
| 存储实验室: | Tsuyoshi Nakagawa |
|
载体骨架基本信息 |
|
| 载体名称: | pPZP221 |
| 载体出品公司: | Hajdukiewicz P., Svab Z. and Maliga P. |
| 载体抗性: | 氯霉素和大观霉素 |
| 空载体大小: | 8500 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 去除庆大霉素耐药标记物和卡那霉素耐药标记物（NOS发起人：NPTII：NOS终止符）已插入。 |
| 载体类型: | 植物表达 |
| 筛选标记: | 卡那霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CaMV35S |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGWB455 was a gift from Tsuyoshi Nakagawa (Addgene plasmid # 74837 ; http://n2t.net/addgene:74837 ; RRID:Addgene_74837) |
| 参考文献部分: | Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants. Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, Ishiguro S. Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7. 10.1271/bbb.70216 PubMed 17690442 |
| 相关质粒: |
pGWB4 pGWB5 pGWB11 pGWB12 pGWB17 pGWB18 pGWB21 pGWB406 pGWB453 pGWB454 pGWB455 pGWB504 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGWB455 12362 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Gateway cloning compatible binary vector for N-terminal fusion with
mRFP (CaMV35S promoter)..
ACCESSION .
VERSION .
KEYWORDS pGWB455
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12362)
AUTHORS Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata
R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T,
Ishiguro S
TITLE Improved Gateway binary vectors: high-performance vectors for
creation of fusion constructs in transgenic analysis of plants.
JOURNAL Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
PUBMED 17690442
REFERENCE 2 (bases 1 to 12362)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12362
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 54..78
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 266..288
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 280..297
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 281..297
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 812..1157
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 1100..1120
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
CDS 1178..1852
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/note="mammalian codon-optimized"
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
primer_bind complement(1308..1326)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1576..1595
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 1770..1793
/label=DsRed1-C
/note="DsRed1, forward primer"
protein_bind 1862..1986
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 2023..2053
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 2107..2766
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(2173..2192)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 3108..3413
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 3292..3311
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(3454..3578)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
terminator 3604..3856
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(3890..3906)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3890..3906)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3903..3925)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3914..3930
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3938..3968)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3983..4004
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
terminator 4038..4290
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(4680..5474)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4791..4810)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 5401..5420
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
promoter complement(5495..5678)
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
promoter complement(5531..5710)
/label=NOS promoter
/note="nopaline synthase promoter"
misc_feature 5884..5908
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 6429..7220
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 7466..8054
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7955..7974
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8208..8225
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 8240..8380
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(8306..8325)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8466..8488
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 8724..8918
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(8984..10057)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(10486..11115)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gcctcctccg aggacgtcat
1201 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
1261 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
1321 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tccagtacgg
1381 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
1441 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
1501 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
1561 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
1621 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa
1681 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
1741 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
1801 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg ccgctgttat
1861 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
1921 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
1981 actatggcgg cccctcgagg ggccgcatta ggcaccccag gctttacact ttatgcttcc
2041 ggctcgtata atgtgtggat tttgagttag gatccggcga gattttcagg agctaaggaa
2101 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
2161 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
2221 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
2281 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
2341 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
2401 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
2461 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
2521 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
2581 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
2641 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
2701 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
2761 gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
2821 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
2881 gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
2941 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
3001 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
3061 tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg
3121 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
3181 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
3241 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
3301 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
3361 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
3421 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
3481 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
3541 ttttacgttt ctcgttcagc tttcttgtac aaagtggtga taacctagag ctcgaatttc
3601 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
3661 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
3721 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
3781 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
3841 tctatgttac tagatcggga attggttccg gaaccaattc gtaatcatgg tcatagctgt
3901 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
3961 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga attaggcgcg
4021 cctatttctg aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta
4081 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat
4141 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
4201 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa
4261 gaaactttat tgccaaatgt ttgaacgatc ggggatcatc cgggtctgtg gcgggaactc
4321 cacgaaaata tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc ctgggcagtc
4381 gccgccgacg ccgttgatgt ggacgccggg cccgatcata ttgtcgctca ggatcgtggc
4441 gttgtgcttg tcggccgttg ctgtcgtaat gatatcggca ccttcgaccg cctgttccgc
4501 agagatcccg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
4561 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
4621 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
4681 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
4741 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
4801 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
4861 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
4921 acgagatcat cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
4981 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
5041 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
5101 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
5161 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
5221 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
5281 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
5341 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
5401 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
5461 tcttgttcaa tcatgcgaaa cgatccagat ccggtgcaga ttatttggat tgagagtgaa
5521 tatgagactc taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac
5581 aagaaatatt tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt
5641 ctgacgtatg tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac
5701 gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca
5761 taacgtgact cccttaattc tccgctcatg atgaattcag aaataaattc agcctaattc
5821 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
5881 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
5941 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
6001 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
6061 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
6121 tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt
6181 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
6241 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
6301 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
6361 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
6421 taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
6481 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
6541 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
6601 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
6661 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
6721 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
6781 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
6841 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
6901 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
6961 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
7021 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
7081 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
7141 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
7201 ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg
7261 gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
7321 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
7381 atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
7441 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
7501 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
7561 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
7621 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
7681 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
7741 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
7801 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
7861 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
7921 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
7981 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
8041 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
8101 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
8161 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
8221 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
8281 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
8341 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
8401 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
8461 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
8521 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg
8581 gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac
8641 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt
8701 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa
8761 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca
8821 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa
8881 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt
8941 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg
9001 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct
9061 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac
9121 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc
9181 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg
9241 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga
9301 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc
9361 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc
9421 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg
9481 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt
9541 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt
9601 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg
9661 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca
9721 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc
9781 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc
9841 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc
9901 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt
9961 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc
10021 cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt
10081 tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt
10141 tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc
10201 gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta
10261 gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca
10321 tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg
10381 tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac
10441 ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga
10501 tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc
10561 aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga
10621 ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt
10681 gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat
10741 gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg
10801 caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg
10861 gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat
10921 cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc
10981 gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag
11041 ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac
11101 agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac
11161 gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg
11221 cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa
11281 ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac
11341 ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg
11401 tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt
11461 cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca
11521 cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag
11581 ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg
11641 ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc
11701 cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg
11761 tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca
11821 ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg
11881 ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc
11941 tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc
12001 agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc
12061 gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct
12121 agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg
12181 gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg
12241 gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac
12301 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
12361 ct
//
